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When applying IEM, i5 sequences has to be entered into these program during the forward orientation in all conditions and for all instrument types.
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The MiniSeq Fast produced a recipe change that takes advantage of the grafted oligo for that i5 index priming, so it's browse from the forward orientation much like a MiSeq.
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Which means that these application Focus on the belief that the i5 sequence is often during the forward orientation and will immediately develop the reverse complement on the sequences furnished for the right instrument form.
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For operates uploaded manually (i.e., an Illumina Instrument is not getting used to produce the sample sheet), the index orientation depends on which instrument you will be making use Ordre mondial of. Please begin to see the Index Orientation Table for info on ways to enter the i5 sequence with regards to the instrument and software program made use of.